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Monday, December 3, 2012

hypothesis in biotechnology (Epigenetic Status of Gdnfin the Ventral Striatum Determines Susceptibility and Adaptation to Daily Stressful Events) this reserch paper




       
if we down regulate the production of gdnf by rnai,then we can potentially decrease  the behavioral response during  chronic stress….. 
this is my hypothesis on the basses  of ( Epigenetic Status of Gdnfin the Ventral Striatum
Determines Susceptibility and Adaptation
to Daily Stressful Events) this reserch paper



Reference: Epigenetic Status ofGdnfin the Ventral Striatum
Determines Susceptibility and Adaptation
to Daily Stressful Events
my Experiment
  1. One is test and other is control
  2. construct a SAV with gdnf gene DOWNSTREAM TO GFP
  3. In test , ADD gdnf-shRNA
  4. In Control, Add Sc-shRNA
  5. Then we use the CUMS (chronic ultra-mild stress )procedures for testing the stress level
  6. Here the CUMS procedure I followed as per my research paper …
Expected result:
The production of gdnf is down regulated

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