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Monday, December 3, 2012

Agarose Gel Electrophoresis

what do you mean by agarose gel electrophoresis ?
            Basically AGE is  separate  the  DNA fragments  base don their Molecular weight.
in AGE DNA molecules are separated on the basis of charge .AGE the applying electric fild determine it .the shoter molecules travel very easily and longer travel slowly
for the visualizing of dna in the gel we are useing ethidlum bromide.
here we are useing   Tris -borate -EDTA(  TBE) buffer to maintain the pH.

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